TRIM54 antibody

Catalog No. ABIN1387738

Product Details anti-TRIM54 Antibody

Target: TRIM54 (Tripartite Motif Containing 54 (TRIM54))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM54 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MURF3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for TRIM54

Target: TRIM54 (Tripartite Motif Containing 54 (TRIM54))

Alternative Name: MURF3 (TRIM54 Products)

Synonyms: MGC80214 antibody, si:dkey-221h15.2 antibody, DKFZp468L1522 antibody, MURF antibody, MURF-3 antibody, RNF30 antibody, muRF3 antibody, 4930486E09Rik antibody, 4930566I02Rik antibody, MuRF3 antibody, Rnf30 antibody, tripartite motif containing 54 antibody, tripartite motif containing 54 S homeolog antibody, tripartite motif-containing 54 antibody, TRIM54 antibody, trim54.S antibody, trim54 antibody, Trim54 antibody

Background:

Synonyms: MURF 3, MURF, MURF-3, MuRF3, Muscle specic RING finger protein 3, Muscle specic RING finger protein, Muscle specic RING finger protein homolog, Muscle-specic RING finger protein 3, Muscle-specic RING finger protein, Ring finger protein 30, RNF30, TRI54_HUMAN, TRIM 54, TRIM54, RNF28, Tripartite mot containing 54, tibody Tripartite mot-containing protein 54.

Background: MuRF1, is a nuclear protein that interacts with SMT3b and the large myofibrillar protein Titin. In muscle cells, MuRF2 (RFN29) regulates gene expression and protein turnover. It localizes to the cytoplasm, but under atrophic conditions it is detected in the nucleus. MuRF2 can form oligomers with various other proteins, including Titin and Myosin. MuRF3, also designated tripartite motif-containing 54 (TRIM54) or ring finger protein 30 (RNF30), interacts with tubulin and stabilizes microtubules duing myotube formation. It is a cytoplasmic protein the localizes to the Z-lines in skeletal muscles, while MuRF2 localizes to the sarcomeric M-band in cardiomyocytes. MuRF3 shares 77 % and 65 % sequence identity with MuRF1 and MuRF2, respectively. MuRF1-3 share a conserved N-terminal RING domain and zinc-binding B-box motif, and two coiled-coil dimerization motif boxes, in their central regions.

Gene ID: 57159