Interferon Induced with Helicase C Domain 1 (IFIH1) antibody

Antigen: Interferon Induced with Helicase C Domain 1 (IFIH1)

Synonyms: Hlcd, MDA5, MDA-5, IDDM19, MGC133047

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185002

Additional Information

Alternative name: MDA5 / IFIH1

Gene ID: 64135

Immunogen: SNGYSTDENFRYL-C

Format: Affinity-purified

Description: All names and symbols: IFIH1, MDA5, MDA-5, Hlcd, MGC133047, IDDM19, melanoma differentiation associated protein-5, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, interferon induced with helicase C domain 1. Official Symbol: IFIH1. RefSeq number(s): NP_071451.2

Specificity: Human 1025 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: No signal obtained yet but low background observed in Human Placenta lysate at up to 1µg/ml. Immunohistochemistry: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining of some cells in the islets of Langerhans. Recommended concentration, 10µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185002 (10µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Publications

Publications: Kang, Gopalkrishnan, Wu et al.: "mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 2, pp. 637-42, 2002 (PubMed).