SIL1 antibody (C-Term)

Catalog No. ABIN185071

This Goat Polyclonal antibody specifically detects SIL1 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for SIL1 antibody (C-Term) (ABIN185071)

Target: SIL1 (Nucleotide Exchange Factor SIL1 (SIL1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SIL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-SIL1 Antibody

Binding Specificity: C-Term

Purpose: BAP / SIL1

Sequence: ELLGSVNSLL KELR

Specificity: Variants (NP_071909.1, NP_001032722.1) encode the same isoform.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ELLGSVNSLLKELR, from the C Terminus of the protein sequence according to NP_071909.1, NP_001032722.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Liver and Testis. Recommended concentration: 5 μg/mL.

Western Blot: Approx 55-60 kDa band observed in Human Liver and Kidney extracts (predicted MW of 53 kDa according to NP_071909.1). Recommended concentration: 1-2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SIL1

Target: SIL1 (Nucleotide Exchange Factor SIL1 (SIL1))

Alternative Name: SIL1

Background: SIL1, BAP, endoplasmic reticulum chaperone SIL1, homolog of yeast, BiP-associated protein, SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae), MSS, ULG5, SIL1 protein

Gene ID: 64374

NCBI Accession: NP_071909, NP_001032722

Pathways: Unfolded Protein Response, SARS-CoV-2 Protein Interactome