SIL1 Homolog, Endoplasmic Reticulum Chaperone (S. Cerevisiae) (SIL1) antibody

Antigen: SIL1 Homolog, Endoplasmic Reticulum Chaperone (S. Cerevisiae) (SIL1)

Synonyms: BAP, MSS, ULG5

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185071

Additional Information

Alternative name: BAP / SIL1

Gene ID: 64374

Immunogen: C-ELLGSVNSLLKELR

Description: All names and symbols: SIL1, BAP, endoplasmic reticulum chaperone SIL1, homolog of yeast, BiP-associated protein. Official Symbol: SIL1. RefSeq number(s): NP_071909.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 55-60 kDa band observed in Human Liver and Kidney extracts (predicted MW of 53kDa according to NP_071909.1). Recommended for use at 1-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185071 staining (1µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Chung, Shen, Hendershot: "BAP, a mammalian BiP-associated protein, is a nucleotide exchange factor that regulates the ATPase activity of BiP." in: The Journal of biological chemistry, Vol. 277, Issue 49, pp. 47557-63, 2002 (PubMed).