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Details for Product No. ABIN185083

SUMO1 Activating Enzyme Subunit 2 (UBA2) antibody

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Antigen
Synonyms ARX, SAE2, Sae2, Uble1b, ATSAE2, EMB2764, EMBRYO DEFECTIVE 2764, F3K23.23, F3K23_23, SUMO-ACTIVATING ENZYME 2, SUMO-activating enzyme 2, sae2, sae2-B, uba2-a, uba2-b, uble1b-B
Reactivity
»Alternatives Human, Mouse (Murine)
Host
Clonality Polyclonal
Application
»Alternatives ELISA
Pubmed 1 reference available
Catalog no. ABIN185083
Quantity 100 µg
Price
261.61 $   Plus shipping costs $45.00
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Immunogen ALSRGLPRELAEA-C
Specificity Human, Mouse
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SAE2 / UBA2
Background All names and symbols: UBA2, SAE2, SUMO-1 activating enzyme subunit 2. Official Symbol: SAE2. RefSeq number(s): NP_005490. Gene ontology terms: ubiquitin-like activating enzyme activity, ubiquitin activating enzyme activity, enzyme activator activity, catalytic activity, ubiquitin cycle
Gene ID 10054, 50995 (mouse)
Research Area Proteolysis / Ubiquitin
Application Notes Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments showed an approx. 35kDa band in Human Testis and Brain extracts at 1ug of primary, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 74KDa according to NP_005490. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any splice variants/modified forms been reported?
Restrictions For Research Use only
Format Liquid
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Preservative Sodium azide
Storage -20 °C
Desterro, Rodriguez, Kemp et al.: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999 (PubMed).

Alternatives for antigen "SUMO1 Activating Enzyme Subunit 2 (UBA2)", type "Antibodies"
Hosts (28), (5), (4)
Reactivities (36), (6), (5), (1), (1)
Applications (32), (22), (15), (7), (4), (2), (1)
Epitopes (8), (4), (2), (1), (1), (1)
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