ArfGAP with RhoGAP Domain, Ankyrin Repeat and PH Domain 3 (ARAP3) antibody

Antigen: ArfGAP with RhoGAP Domain, Ankyrin Repeat and PH Domain 3 (ARAP3)

Synonyms: DRAG1, CENTD3, FLJ21065, Drag1, Centd3, AI851258, KIAA4097, mKIAA4097, E030006K04Rik, ARAP3, RGD1304938

Clonality: Polyclonal

Host: Goat

Reactivity: Please enquire

Application: ELISA

Catalog no.: ABIN185087

Additional Information

Alternative name: CENTD3 / ARAP3

Gene ID: 64411

Immunogen: CTSSPPSSQPLT

Description: All names and symbols: CENTD3, centaurin, delta 3, ARAP3, FLJ21065, ARF-GAP, RHO-GAP, ankyrin repeat and plekstrin homology domains-containing protein 3, PtdIns(3,4,5)P3-binding protein, phosphoinositide binding protein. Official Symbol: CENTD3. RefSeq number(s): NP_071926. Gene ontology terms: DNA binding, neuropeptide signaling pathway, nucleus

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4000. Western Blot: Preliminary experiments gave no signal but low background in Human Heart and Jurkat extracts at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Krugmann, Anderson, Ridley et al.: "Identification of ARAP3, a novel PI3K effector regulating both Arf and Rho GTPases, by selective capture on phosphoinositide affinity matrices." in: Molecular cell, Vol. 9, Issue 1, pp. 95-108, 2002 (PubMed).