ArfGAP with RhoGAP Domain, Ankyrin Repeat and PH Domain 1 (ARAP1) antibody

Antigen: ArfGAP with RhoGAP Domain, Ankyrin Repeat and PH Domain 1 (ARAP1)

Synonyms: Centd2, MGC176140, mKIAA0782, 2410002L19Rik, CENTD2, KIAA0782, ARAP1, centd2, Ci-ArfGAP-8

Clonality: Polyclonal

Host: Goat

Reactivity: Please enquire

Application: ELISA

Catalog no.: ABIN185099

Additional Information

Alternative name: CENTD2 / ARAP1

Gene ID: 116985, 69710 (mouse)

Immunogen: C-AFTADPLSLLRNV

Description: All names and symbols: CENTD2, centaurin, delta 2, ARAP1, KIAA0782, ARF-GAP, RHO-GAP, ankyrin repeat, and pleckstrin homology domains-containing protein 1. Official Symbol: CENTD2. RefSeq number(s): NP_056057, NP_631920. Gene ontology terms: DNA binding, neuropeptide signaling pathway, nucleus

Specificity: This antibody is expected to recognise both reported isoforms (represented by NP_056057 and NP_631920). Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave no signal but low background in Human Skeletal Muscle and Lung extracts at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Miura, Jacques, Stauffer et al.: "ARAP1: a point of convergence for Arf and Rho signaling." in: Molecular cell, Vol. 9, Issue 1, pp. 109-19, 2002 (PubMed).