PIK3C2A antibody (C-Term)

Catalog No. ABIN185194

This Goat Polyclonal antibody specifically detects PIK3C2A in IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for PIK3C2A antibody (C-Term) (ABIN185194)

Target: PIK3C2A (Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PIK3C2A antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-PIK3C2A Antibody

Binding Specificity: C-Term

Purpose: PIK3C2A

Sequence: TVKWYQLTAA TYL

Cross-Reactivity: Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TVKWYQLTAATYL, from the C Terminus of the protein sequence according to NP_002636.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Placenta shows cytoplasm staining of cytotrophoblasts and mesenchymal cells. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.

Western Blot: Preliminary experiments gave no signal but low background in Human Testis and Molt-4 lysates at up to 1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PIK3C2A

Target: PIK3C2A (Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A))

Alternative Name: PIK3C2A

Background: PIK3C2A, CPK, PI3-K-C2A, PI3K-C2alpha, PI3-K-C2(ALPHA), phosphoinositide-3-kinase, class 2, alpha polypeptide, C2-containing phosphatidylinositol kinase, DKFZp686L193, MGC142218,

Gene ID: 5286, 18704

NCBI Accession: NP_002636

Pathways: EGFR Signaling Pathway, Inositol Metabolic Process, Platelet-derived growth Factor Receptor Signaling