ARP1 Homolog B antibody

Antigen: ARP1 Homolog B

Synonyms: PC3, ARP1B, CTRN2, Arp1b, AA960180, AI851923, MGC36526, 2310066K23Rik, MGC116310, Actr1b, zehn1110, MGC113996, wu:fc22g09, wu:fc56d01, wu:fy52e08, hm:zehn1110, ACTR1B, MGC139536

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185220

Additional Information

Gene ID: 10120, 226977 (mouse)

Immunogen: Peptide with sequence C-YEEDGSRAIHRKT, from the C Terminus of the protein sequence according to NP_005726.1

Format: Affinity-purified

Description: All names and symbols: ACTR1B, ARP1B, CTRN2, ARP1 actin-related protein 1 homolog B, centractin beta (yeast), PC3, centractin beta, ARP1, yeast homolog B, ARP1 (actin-related protein 1, yeast) homolog B (centractin beta), ARP1 actin-related protein 1 homolog B, centractin beta. Official Symbol: ACTR1B. RefSeq number(s): NP_005726.1. Gene ontology terms: microtubule associated complex, dynactin complex This epitope was selected to minimise the chance of cross-reactivity with ACTR1A (YEEDGARSIHRKT)..

Specificity: Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 45kDa band observed in human heart and brain lysates (predicted MW of 43kDa according to NP_005726). Recommended for use at 0.5-2µg/ml. Immunohistochemistry: In paraffin embedded Human Uterus shows fibrillar staining of cytoplasm in endometrial cells. Recommended concentration, 3-5µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Clark, Staub, Clark et al.: "Beta-centractin: characterization and distribution of a new member of the centractin family of actin-related proteins." in: Molecular biology of the cell, Vol. 5, Issue 12, pp. 1301-10, 1995 (PubMed).