ACTR1B antibody (C-Term)

Catalog No. ABIN2784773

Cited in 1 publication. The Rabbit Polyclonal anti-ACTR1B antibody (ABIN2784773) specifically detects ACTR1B in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Rabbit, Guinea Pig, Horse and Zebrafish (Danio rerio) samples.

Quick Overview for ACTR1B antibody (C-Term) (ABIN2784773)

Target: ACTR1B (ARP1 Actin-Related Protein 1 Homolog B, Centractin beta (ACTR1B))

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTR1B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ACTR1B Antibody

Binding Specificity: C-Term

Purpose: ACTR1B Antibody - C-terminal region

Sequence: KIKISAPQER LYSTWIGGSI LASLDTFKKM WVSKKEYEED GSRAIHRKTF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against ACTR1B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ACTR1B

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8094976

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-ACTR1B antibody (ABIN2784773)

Hwang, Weng, Lin, Bau, Ko, Tsai, Tsai, Wu, Lin, Huang, Kuo: "Hypoxia-induced compensatory effect as related to Shh and HIF-1alpha in ischemia embryo rat heart." in: Molecular and cellular biochemistry, Vol. 311, Issue 1-2, pp. 179-87, (2008) (PubMed).

Target Details for ACTR1B

Target: ACTR1B (ARP1 Actin-Related Protein 1 Homolog B, Centractin beta (ACTR1B))

Alternative Name: ACTR1B

Background:

Background Information: ACTR1B is a 42.3 kD subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. ACTR1B, like ACTR1A, is an actin-related protein. These two proteins, which are of equal length and share 90 % amino acid identity, are present in a constant ratio of approximately 1:15 in the dynactin complex.This gene encodes a 42.3 kD subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like ACTR1A, is an actin-related protein. These two proteins are of equal length and share 90 % amino acid identity. They are present in a constant ratio of approximately 1:15 in the dynactin complex.

Gene Name: ARP1 actin-related protein 1 homolog B, centractin beta (yeast)

Alternative Symbols: PC3, ARP1B, CTRN2

Protein Name: Beta-centractin

Molecular Weight: 42kDa

Gene ID: 10120

NCBI Accession: NP_005726

UniProt: P42025