ARPC3 antibody (C-Term)

Catalog No. ABIN185223

This anti-ARPC3 antibody is a Goat Polyclonal antibody detecting ARPC3 in WB and ELISA. Suitable for Human and Mouse. This Primary Antibody has been cited in 1 publication.

Quick Overview for ARPC3 antibody (C-Term) (ABIN185223)

Target: ARPC3 (Actin Related Protein 2/3 Complex, Subunit 3, 21kDa (ARPC3))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This ARPC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-ARPC3 Antibody

Binding Specificity: C-Term

Purpose: ARPC3

Sequence: RQFMNKSLSG PGQ

Specificity: Please note there are two hypothetical proteins called "similar to the Arp2/3 protein complex subunit p21-Arc" that are virtually identical (CAC14083, XP_208062).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RQFMNKSLSGPGQ, from the C Terminus of the protein sequence according to NP_005710.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Band observed just below 20 kDa marker in HeLa, Human Brain and Mouse Brain lysates (predicted MW of 20 kDa according to NP_005710). Recommended for use at 1-3 μg/mL. Primary incubation was for 1 hour. This antibody has been successfully used

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-ARPC3 antibody (ABIN185223)

Fu, Brown, Yap, Winckler, Jaiswal, Liu: "Doublecortin (Dcx) family proteins regulate filamentous actin structure in developing neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 2, pp. 709-21, (2013) (PubMed).

Target Details for ARPC3

Target: ARPC3 (Actin Related Protein 2/3 Complex, Subunit 3, 21kDa (ARPC3))

Alternative Name: ARPC3

Background: ARPC3, ARC21, p21-Arc, actin related protein 2/3 complex, subunit 3, 21 kDa, ARP2/3 protein complex subunit p21, actin related protein 2/3 complex subunit 3

Gene ID: 10094, 56378

NCBI Accession: NP_005710

Pathways: RTK Signaling, Regulation of Actin Filament Polymerization