Actin Related Protein 2/3 Complex, Subunit 3, 21kDa (ARPC3) antibody

Antigen: Actin Related Protein 2/3 Complex, Subunit 3, 21kDa (ARPC3)

Synonyms: ARC21, p21-Arc, 21kDa, p21-Ar, p21Arc, AA408672, AI788639, 1110006A04Rik, MGC68983, MGC127675, arc21, p21-arc

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185223

Additional Information

Alternative name: ARPC3

Gene ID: 10094, 56378 (mouse)

Immunogen: C-RQFMNKSLSGPGQ

Format: Affinity-purified

Description: All names and symbols: ARPC3, ARC21, p21-Arc, actin related protein 2/3 complex, subunit 3, 21kDa, ARP2/3 protein complex subunit p21. Official Symbol: ARPC3. RefSeq number(s): NP_005710. Gene ontology terms: structural constituent of cytoskeleton, cell motility, Arp2/3 protein complex, actin cytoskeleton

Specificity: Please note there are two hypothetical proteins called similar to the Arp2/3 protein complex subunit p21-Arc that are virtually identical (CAC14083, XP_208062). Human, Mouse, Rat 178 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Band observed just below 20kDa marker in Hela, Human Brain and Mouse Brain lysates (predicted MW of 20kDa according to NP_005710). Recommended for use at 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185223 staining (1µg/ml) of Mouse Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Welch, DePace, Verma et al.: "The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly." in: The Journal of cell biology, Vol. 138, Issue 2, pp. 375-84, 1997 (PubMed).