Unc-13 Homolog D (C. Elegans) (UNC13D) (C-Term) antibody

Antigen: Unc-13 Homolog D (C. Elegans) (UNC13D)

Synonyms: FHL3, HLH3, HPLH3, Munc13-4, Jinx, FLJ00067, mFLJ00067, RP23-42F6.5, 2610108D09Rik, Unc13h4, UNC13D

Binding Site: C-Term

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185243

Additional Information

Alternative name: Munc13-4 / UNC13D

Gene ID: 201294

Immunogen: C-KQASQHALRPAP

Description: All names and symbols: UNC13D, Munc13-4 , unc-13 homolog D (C. elegans) , FHL3, HLH3, HPLH3, unc-13 homolog D. Official Symbol: UNC13D. RefSeq number(s): NP_954712.1

Specificity: Human 1090 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx. 105kDa band observed in Human T-lymphocyte and HeLa lysates (calculated MW of 123kDa according to NP_954712.1). Recommended concentration: 1-3µg/ml. Full caption: ABIN185243 (2µg/ml) staining of Human T-lymphocyte lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data provided by anonymous customer.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185243 (2µg/ml) staining of Human T-lymphocyte lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Feldmann, Callebaut, Raposo et al.: "Munc13-4 is essential for cytolytic granules fusion and is mutated in a form of familial hemophagocytic lymphohistiocytosis (FHL3)." in: Cell, Vol. 115, Issue 4, pp. 461-73, 2003 (PubMed).