Deleted in Liver Cancer 1/3 (DLC1/3) antibody

Antigen: Deleted in Liver Cancer 1/3 (DLC1/3)

Synonyms: HP, ARHGAP7, STARD12, FLJ21120, p122-RhoGAP, rhogap7, MGC83845, MGC152430

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185264

Additional Information

Alternative name: DLC1 (Isoforms 1 and 3)

Gene ID: 10395, 50768 (mouse)

Immunogen: SVAIRKRSWEEHC

Format: Affinity-purified

Description: All names and symbols: DLC1, HP, ARHGAP7, STARD12, FLJ21120, p122-RhoGAP, deleted in liver cancer 1, Rho-GTPase-activating protein 7, START domain containing protein 12, StAR-related lipid transfer protein 12. Official Symbol: DLC1. RefSeq number(s): NP_872584.1, NP_079043.2. Gene ontology terms: Rho GTPase activator activity, cytoplasm, cytoskeleton organization and biogenesis, negative regulation of cell growth, protein binding, regulation of cell adhesion

Specificity: This antibody is expected to recognise isoforms 1 and 3 ( NP_872584.1 and NP_079043.2 resp) but NOT isoform 2 (NP_006085.2). Human, Mouse 1091 aa, 498 aa, 1528 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 28kDa band in Human Liver and Lung lysates as well as in different epithelial lung cell lines after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 171 kDa according to NP_872584.1 and 54.6kDa according to NP_079043.2 (isotope 3). The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Adrenal Gland (Medulla) shows strong reticulate staining in the cytoplasm of medular cells. Recommended concentration, 5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185264 (5µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Wilson, McGlinn, Marsh et al.: "Sequence variants of DLC1 in colorectal and ovarian tumours." in: Human mutation, Vol. 15, Issue 2, pp. 156-65, 2000 (PubMed).