Resistance To Inhibitors of Cholinesterase 8 Homolog A (C. Elegans) (RIC8A) antibody

Antigen: Resistance To Inhibitors of Cholinesterase 8 Homolog A (C. Elegans) (RIC8A)

Synonyms: RIC8, MGC104517, MGC131931, MGC148073, MGC148074, synembryn, RIC-8, Ric8a, AI114950, Ric8, Ric-8A, ric8, MGC76200, zgc:92294, MGC139893, DKFZp468C139, Syn, ric8a, CG15797, DmelCG15797, DmRic8, l(1)G0397, ric-8, Ric-8, ric-8a, RIC8A, ricc8aa

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185305

Additional Information

Alternative name: RIC8A / Synembryn

Gene ID: 60626, 101489 (mouse)

Immunogen: CLSSDPDSDPD

Format: Affinity-purified

Description: All names and symbols: RIC8A, synembryn, resistance to inhibitors of cholinesterase 8 homolog A (C. elegans), MGC104517, MGC131931, RIC8, RIC8 protein, resistance to inhibitors of cholinesterase 8 homolog A. Official Symbol: RIC8A. RefSeq number(s): NP_068751.4

Specificity: Human, Mouse, Rat 537 aa

Synonyms: RIC8, MGC104517, MGC131931, MGC148073, MGC148074, synembryn, RIC-8, Ric8a, AI114950, Ric8, Ric-8A, ric8, MGC76200, zgc:92294, MGC139893, DKFZp468C139, Syn, ric8a, CG15797, DmelCG15797, DmRic8, l(1)G0397, ric-8, Ric-8, ric-8a, RIC8A, ricc8aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 70kDa band observed in human brain lysates (calculated MW of 60.4kDa according to NP_068751.4). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185305 staining (1µg/ml) of human brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Tall, Krumins, Gilman: "Mammalian Ric-8A (synembryn) is a heterotrimeric Galpha protein guanine nucleotide exchange factor." in: The Journal of biological chemistry, Vol. 278, Issue 10, pp. 8356-62, 2003 (PubMed).

Klattenhoff, Montecino, Soto et al.: "Human brain synembryn interacts with Gsalpha and Gqalpha and is translocated to the plasma membrane in response to isoproterenol and carbachol." in: Journal of cellular physiology, Vol. 195, Issue 2, pp. 151-7, 2003 (PubMed).

Afshar, Willard, Colombo et al.: "RIC-8 is required for GPR-1/2-dependent Galpha function during asymmetric division of C. elegans embryos." in: Cell, Vol. 119, Issue 2, pp. 219-30, 2004 (PubMed).