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Peptidyl Arginine Deiminase, Type IV (PADI4) antibody

Antigen

Peptidyl Arginine Deiminase, Type IV (PADI4)

Synonyms Pdi4, PAD, PAD4, PDI4, PDI5, PADI5
Clonality Polyclonal
Host
Reactivity
Alternatives

Human

Application
Alternatives ELISA, Western Blotting (WB)
1 reference available
Catalog no. ABIN185308
Quantity 100 ug
Price 501.80 $   Plus shipping costs $35.00
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Additional Information

Alternative name PAD4 / PADI4
Gene ID 23569
Immunogen AQGTLIRVTPEQPC
Format Affinity-purified
Description All names and symbols: PADI4, PAD, PDI4, PDI5, PADI5, peptidyl arginine deiminase, type IV, protein-arginine deiminase, peptidyl arginine deiminase, type V. Official Symbol: PADI4. RefSeq number(s): NP_036519. Gene ontology terms: calcium ion binding, hydrolase activity, protein modification, protein-arginine deiminase activity
Specificity Human, Chimpanzee 663 aa

Application Details

Application Notes Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 74kDa band observed in human spleen and human liver lysates (calculated MW of 74.1kDa according to NP_036519). Recommended concentration: 0.3-2µg/ml.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions For Research Use only

Publications

Publications Wang, Wysocka, Sayegh et al.: "Human PAD4 regulates histone arginine methylation levels via demethylimination." in: Science (New York, N.Y.), Vol. 306, Issue 5694, pp. 279-83, 2004 (PubMed).

Alternatives

Alternatives for antigen "Peptidyl Arginine Deiminase, Type IV (PADI4)", type "Antibodies"
Hosts Rabbit (35), Mouse (4), Goat (3)
Reactivities Human (40), Mouse (Murine) (3), Rat (Rattus) (2), Chimpanzee (1)
Applications Western Blotting (WB) (24), Flow Cytometry (FACS) (20), Immunofluorescence (IF) (20), Enzyme Immunoassay (EIA) (11), ELISA (8), Immunoprecipitation (IP) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitopes N-Term (3), C-Term (2), Center (1)