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Antibodies
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anti-Peptidyl Arginine Deiminase, Type IV (PADI4) antibody
Peptidyl Arginine Deiminase, Type IV (PADI4) antibody
| Antigen |
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| Synonyms |
Pdi4, PAD, PAD4, PDI4, PDI5, PADI5 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Western Blotting (WB) (24), Flow Cytometry (FACS) (20), Immunofluorescence (IF) (20), Enzyme Immunoassay (EIA) (11), ELISA (8), Immunoprecipitation (IP) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN185308 |
| Quantity |
100 ug |
| Price |
501.80 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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PAD4 / PADI4
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Gene ID
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23569
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Immunogen
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AQGTLIRVTPEQPC
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Format
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Affinity-purified
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Description
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All names and symbols: PADI4, PAD, PDI4, PDI5, PADI5, peptidyl arginine deiminase, type IV, protein-arginine deiminase, peptidyl arginine deiminase, type V. Official Symbol: PADI4. RefSeq number(s): NP_036519. Gene ontology terms: calcium ion binding, hydrolase activity, protein modification, protein-arginine deiminase activity
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Specificity
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Human, Chimpanzee 663 aa
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 74kDa band observed in human spleen and human liver lysates (calculated MW of 74.1kDa according to NP_036519). Recommended concentration: 0.3-2µg/ml.
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Restrictions
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For Research Use only
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Publications
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Wang, Wysocka, Sayegh et al.: "Human PAD4 regulates histone arginine methylation levels via demethylimination." in: Science (New York, N.Y.), Vol. 306, Issue 5694, pp. 279-83, 2004 (PubMed).
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Alternatives for antigen "Peptidyl Arginine Deiminase, Type IV (PADI4)", type "Antibodies"
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Hosts
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Rabbit (35), Mouse (4), Goat (3)
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Reactivities
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Human (40), Mouse (Murine) (3), Rat (Rattus) (2), Chimpanzee (1)
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Applications
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Western Blotting (WB) (24), Flow Cytometry (FACS) (20), Immunofluorescence (IF) (20), Enzyme Immunoassay (EIA) (11), ELISA (8), Immunoprecipitation (IP) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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N-Term (3), C-Term (2), Center (1)
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