Mitogen-Activated Protein Kinase 1 (MAPK1) antibody

Antigen: Mitogen-Activated Protein Kinase 1 (MAPK1)

Synonyms: ERK, p38, p40, p41, ERK2, ERT1, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk, ERK3, PRKM6, p97MAPK, HsT17250, DKFZp686F03189, Erk2, Prkm1, C78273, p42mapk, AA407128, AU018647, 9030612K14Rik, zERK2, MAPK1, Xp42, erk, erk2, mapk, mpk1, xp42, mapk1a, MGC68934, ert1, mapk2, prkm1, prkm2, F14N23.9, F14N23_9, MITOGEN-ACTIVATED PROTEIN KINASE, MITOGEN-ACTIVATED PROTEIN KINASE 1, MPK1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185327

Additional Information

Alternative name: ERK2 / MAPK1

Gene ID: 5594, 26413 (mouse)

Immunogen: CAAGPEMVRGQVF

Description: All names and symbols: MAPK1, ERK2, ERK, p38, p40, p41, ERT1, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk, mitogen-activated protein kinase 1, protein tyrosine kinase ERK2, mitogen-activated protein kinase 2, extracellular signal-regulated kinase 2. Official Symbol: MAPK1. RefSeq number(s): NP_002736, NP_620407. Gene ontology terms: cyclin, DNA replication, regulation of cell cycle

Specificity: This antibody is expected to recognise an epitope corresponding to aa 9-19 of both reported protein isoforms of human ERK2 / MAPK1 protein. Human, Mouse, Chimp, Bull, Chicken, Xenopus

Synonyms: ERK, p38, p40, p41, ERK2, ERT1, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk, ERK3, PRKM6, p97MAPK, HsT17250, DKFZp686F03189, Erk2, Prkm1, C78273, p42mapk, AA407128, AU018647, 9030612K14Rik, zERK2, MAPK1, Xp42, erk, erk2, mapk, mpk1, xp42, mapk1a, MGC68934, ert1, mapk2, prkm1, prkm2, F14N23.9, F14N23_9, MITOGEN-ACTIVATED PROTEIN KINASE, MITOGEN-ACTIVATED PROTEIN KINASE 1, MPK1

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 38kDa band observed in human cervix epitheloid carcinoma HeLa and and human hepatoblastoma HepG2 lysates (calculated MW of 41.4kDa according to NP_002736 and NP_620407). Recommended concentration: 0.5-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185327 (0.5µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Favata, Horiuchi, Manos et al.: "Identification of a novel inhibitor of mitogen-activated protein kinase kinase." in: The Journal of biological chemistry, Vol. 273, Issue 29, pp. 18623-32, 1998 (PubMed).

Lee, Nguyen, Park et al.: "EPO receptor-mediated ERK kinase and NF-kappaB activation in erythropoietin-promoted differentiation of astrocytes." in: Biochemical and biophysical research communications, Vol. 320, Issue 4, pp. 1087-95, 2004 (PubMed).