Glutathione S-Transferase mu 1/2 (GSTM1/2) antibody

Antigen: Glutathione S-Transferase mu 1/2 (GSTM1/2)

Synonyms: MU, H-B, GST1, GTH4, GTM1, MU-1, GSTM1-1, MGC26563, GSTM1a-1a, GSTM1b-1b

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185334

Additional Information

Alternative name: GSTM1 / GSTM2

Gene ID: 2944, 29

Immunogen: CVFSKMAVWGNK

Format: Affinity-purified

Description: All names and symbols: GSTM1, GSTM2, MU, H-B, GST1, GTH4, GTM1, MU-1, GSTM1-1, MGC26563, GSTM1a-1a, GSTM1b-1b, glutathione S-transferase M1, HB subunit 4, GST class-mu 1, glutathione S-aryltransferase, glutathione S-alkyltransferase, glutathione S-transferase, Mu-1, glutathione S-aralkyltransferase, S-(hydroxyalkyl)glutathione lyase. Official Symbol: GSTM1 / GSTM2. RefSeq number(s): NP_000552, NP_666533, NP_000839

Specificity: This antibody is expected to recognise both reported variants of human GSTM1 protein (NP_000552, NP_666533) and wil also recognise human GSTM2 protein (NP_000848). Human, Chimp

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:1,000. Western Blot: Approx 26kDa band observed in Human Liver and Human Lung lysates (calculated MW of 25.7kDa according to NP_000552.2, and NP_000839.1). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185334 (0.3µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Seidegard, Pero, Markowitz et al.: "Isoenzyme(s) of glutathione transferase (class Mu) as a marker for the susceptibility to lung cancer: a follow up study." in: Carcinogenesis, Vol. 11, Issue 1, pp. 33-6, 1990 (PubMed).

Seidegard, Pero, Miller et al.: "A glutathione transferase in human leukocytes as a marker for the susceptibility to lung cancer." in: Carcinogenesis, Vol. 7, Issue 5, pp. 751-3, 1986 (PubMed).

Lee, Kim, Woo et al.: "Increased frequencies of glutathione S-transferase (GSTM1 and GSTT1) gene deletions in Korean patients with acquired aplastic anemia." in: Blood, Vol. 98, Issue 12, pp. 3483-5, 2001 (PubMed).

Habalova, Salagovic, Kalina et al.: "Combined analysis of polymorphisms in glutathione S-transferase M1 and microsomal epoxide hydrolase in lung cancer patients." in: Neoplasma, Vol. 51, Issue 5, pp. 352-7, 2005 (PubMed).