»
Antibodies
»
anti-Choline O-Acetyltransferase (CHAT) antibody
Choline O-Acetyltransferase (CHAT) antibody
| Antigen |
|
| Synonyms |
CMS1A, CMS1A2, B230380D24Rik, CT23399, Cat, ChAT, cha, dChAT, l(3)91Cc, DmelCG12345, CG12345, CHAT, ChoAcTase, Ci-ChAT |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
Human (44), Rat (Rattus) (37), Mouse (Murine) (35), Dog (Canine) (19), Pig (Porcine) (18), Guinea Pig (11), Chicken (4), Rabbit (4), Bird (Avian) (2), Cow (Bovine) (2), Horse (Equine) (2), Primates (2), Cat (Feline) (1), Monkey (1)
|
|
Application
|
Western Blotting (WB) (40), Immunohistochemistry (IHC) (26), Immunofluorescence (IF) (20), Flow Cytometry (FACS) (18), Immunohistochemistry (Frozen Sections) (IHC (fro)) (16), ELISA (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Enzyme Immunoassay (EIA) (5), Immunocytochemistry (ICC) (4), Immunoprecipitation (IP) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (EM) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) (1)
|
| Catalog no. |
ABIN185344 |
| Quantity |
100 ug (Variants) |
| Price |
501.80 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 Business Days |
|
Alternative name
|
Choline acetyltransferase
|
|
Gene ID
|
1103
|
|
Immunogen
|
CKEKATRPSQGHQP
|
|
Format
|
Affinity-purified
|
|
Description
|
All names and symbols: choline acetyltransferase, CHAT, CMS1A, CMS1A2, acetyl CoA:choline O-acetyltransferase, CHAT. Official Symbol: CHAT. RefSeq number(s): NP_065574, NP_066264, NP_066265, NP_066266
|
|
Specificity
|
This antibody is expected to recognise both reported isoforms. NP_066264, NP_066265 and NP_066266 are identical proteins representing isoform 1. Human, Pig, Dog, Chimpanzee
|
|
Application Notes
|
Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 70kDa band observed in Human Placenta lysates (calculated MW of 70.5kDa according to NP_065574 and NP_066264). Recommended concentration: 0.03-0.3µg/ml.
|
|
Purification
|
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
|
|
Buffer
|
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
|
|
Storage
|
Aliquot and store at -20°C. Minimize freezing and thawing.
|
|
Restrictions
|
For Research Use only
|
|
Publications
|
Madziar, Lopez-Coviella, Zemelko et al.: "Regulation of cholinergic gene expression by nerve growth factor depends on the phosphatidylinositol-3'-kinase pathway." in: Journal of neurochemistry, Vol. 92, Issue 4, pp. 767-79, 2005 (PubMed).
|
Alternatives for antigen "Choline O-Acetyltransferase (CHAT)", type "Antibodies"
|
Hosts
|
Rabbit (43), Goat (14), Sheep (7), Mouse (6), Chicken (5)
|
|
Reactivities
|
Human (44), Rat (Rattus) (37), Mouse (Murine) (35), Dog (Canine) (19), Pig (Porcine) (18), Guinea Pig (11), Chicken (4), Rabbit (4), Bird (Avian) (2), Cow (Bovine) (2), Horse (Equine) (2), Primates (2), Cat (Feline) (1), Monkey (1)
|
|
Applications
|
Western Blotting (WB) (40), Immunohistochemistry (IHC) (26), Immunofluorescence (IF) (20), Flow Cytometry (FACS) (18), Immunohistochemistry (Frozen Sections) (IHC (fro)) (16), ELISA (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Enzyme Immunoassay (EIA) (5), Immunocytochemistry (ICC) (4), Immunoprecipitation (IP) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (EM) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) (1)
|
|
Conjugates
|
Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
|
|
Epitopes
|
C-Term (2), internal (2), N-Term (1)
|
Alternatives 
