Choline Acetyltransferase antibody (AA 101-200)

Catalog No. ABIN724070

Product Details anti-Choline Acetyltransferase Antibody

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Binding Specificity: AA 101-200

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Choline Acetyltransferase antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Dog, Human, Mouse, Rat

Predicted Reactivity: Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ChAT

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Choline Acetyltransferase antibody (ABIN724070)

Zhang, Zheng, Geng, Xue, Wang, Xie, Wang, Zhang, Hou: "MicroRNA profiling of atrial fibrillation in canines: miR-206 modulates intrinsic cardiac autonomic nerve remodeling by regulating SOD1." in: PLoS ONE, Vol. 10, Issue 3, pp. e0122674, (2015) (PubMed).

Wieck, El-Nachef, Hou, Spurrier, Holoyda, Schall, Garcia Mojica, Collins, Trecartin, Cheng, Frykman, Grikscheit: "Human and Murine Tissue-Engineered Colon Exhibit Diverse Neuronal Subtypes and Can Be Populated by Enteric Nervous System Progenitor Cells When Donor Colon Is Aganglionic." in: Tissue engineering. Part A, (2015) (PubMed).

Zhang, Jin, Li, Guan, Han, Zhang: "Catalpol improves cholinergic function and reduces inflammatory cytokines in the senescent mice induced by D-galactose." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 58, pp. 50-5, (2013) (PubMed).

Zhu, Zhao, Luo, Li: "Gastrointestinal dysfunction in a Parkinson's disease rat model and the changes of dopaminergic, nitric oxidergic, and cholinergic neurotransmitters in myenteric plexus." in: Journal of molecular neuroscience : MN, Vol. 47, Issue 1, pp. 15-25, (2012) (PubMed).

Target Details for Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Alternative Name: ChAT (CHAT Products)

Synonyms: CHOACTASE antibody, CMS1A antibody, CMS1A2 antibody, ChoAcTase antibody, B230380D24Rik antibody, choline O-acetyltransferase antibody, choline O-acetyltransferase a antibody, choline acetyltransferase antibody, carnitine O-acetyltransferase antibody, CHAT antibody, chata antibody, Chat antibody, PGTG_00551 antibody, LOC100533322 antibody

Background:

Synonyms: CMS1A, CMS1A2, CHOACTASE, Choline O-acetyltransferase, ChAT, Choline acetylase

Background: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

Gene ID: 1103

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development