phosphodiesterase 5A, CGMP-Specific (PDE5A) antibody

Antigen: phosphodiesterase 5A, CGMP-Specific (PDE5A)

Synonyms: CN5A, PDE5, PDE5A1, CGB-PDE, PDE5A2, Cn5n, Pde5, Cgbpde, Pde5a1, PDE5A, cn5a, pde5, pde5a1, cgb-pde, MGC146818

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185350

Additional Information

Alternative name: PDE5A

Gene ID: 8654

Immunogen: C-LINGESGQAKRN

Format: Affinity-purified

Description: All names and symbols: PDE5A, CN5A, PDE5, PDE5A1, CGB-PDE, phosphodiesterase 5A, cGMP-specific, cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase. Official Symbol: PDE5A. RefSeq number(s): NP_001074, NP_237223, NP_246273, (NP_236914). Gene ontology terms: 3',5'-cGMP binding, 3',5'-cyclic-nucleotide phosphodiesterase activity, 3',5'-cyclic-nucleotide phosphodiesterase activity, catalytic activity, cellular_component unknown, cyclic nucleotide metabolism, hydrolase activity, magnesium ion binding, signal transduction, signal transduction, zinc ion binding

Specificity: This antibody is expected to recognise all 4 reported isoforms (as represented by NP_001074, NP_236914, NP_246273). NP_237223 has been retracted (280905) Human, Dog, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 100kDa band observed in human lung lysates (calculated MW of 100kDa according to NP_001074). Recommended concentration: 0.3-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer, Hypertrophy, Atherosclerosis, Enzymes, Metabolism

Restrictions: For Research Use only

Images

ABIN185350 staining (0.3µg/ml) of human lung lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Morelli, Filippi, Mancina et al.: "Androgens regulate phosphodiesterase type 5 expression and functional activity in corpora cavernosa." in: Endocrinology, Vol. 145, Issue 5, pp. 2253-63, 2004 (PubMed).