PDE5A antibody (C-Term)

Catalog No. ABIN2789423

Cited in 1 publication. The Rabbit Polyclonal anti-PDE5A antibody (ABIN2789423) specifically detects PDE5A in WB and IHC. The antibody is reactive with Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio) samples.

Quick Overview for PDE5A antibody (C-Term) (ABIN2789423)

Target: PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))

Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE5A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PDE5A Antibody

Binding Specificity: C-Term

Purpose: PDE5A Antibody - C-terminal region

Sequence: TACDLSAITK PWPIQQRIAE LVATEFFDQG DRERKELNIE PTDLMNREKK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Zebrafish: 82%

Characteristics: This is a rabbit polyclonal antibody against PDE5A. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP63250-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-PDE5A antibody (ABIN2789423)

Ioki, Wu, Takayama, Motohashi, Wakai, Funahashi: "A phosphodiesterase type-5 inhibitor, sildenafil, induces sperm capacitation and penetration into porcine oocytes in a chemically defined medium." in: Theriogenology, Vol. 85, Issue 3, pp. 428-33, (2016) (PubMed).

Target Details for PDE5A

Target: PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))

Alternative Name: PDE5A

Background:

Background Information: This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms.

Gene Name: Phosphodiesterase 5A, cGMP-specific

Alternative Symbols: CN5A, PDE5, CGB-PDE

Protein Name: cGMP-specific 3',5'-cyclic phosphodiesterase

Molecular Weight: 93kDa

Gene ID: 8654

NCBI Accession: NP_246273

UniProt: O76074

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling