A Kinase (PRKA) Anchor Protein (Yotiao) 9 (AKAP9) antibody

Antigen: A Kinase (PRKA) Anchor Protein (Yotiao) 9 (AKAP9)

Synonyms: PRKA9, CG-NAP, YOTIAO, AKAP350, AKAP450, HYPERION, KIAA0803, MU-RMS-40.16A, AKAP-9, C79026, AW545847, mKIAA0803, 5730481H23Rik, Gisp, Yotiao, AKAP9, wu:fj02h02, prka9, yotiao, akap350, akap450, hyperion, AKAP18, Akap7, Akap15, Akap18, AKAP18d, MGC112559, AKAP15, AI662165, BB170514, 6430401D08, akap15, akap18, MGC83920, AKAP7, MGC139521

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185354

Additional Information

Alternative name: AKAP9 / AKAP450 / CG-NAP

Gene ID: 10142

Immunogen: C-SGSTTQFHAGMR

Description: All names and symbols: AKAP9, CG-NAP, PRKA9, YOTIAO, AKAP350, HYPERION, KIAA0803, A kinase (PRKA) anchor protein (yotiao) 9, AKAP120-like protein, kinase N-associated protein, A-kinase anchoring protein 450, A-kinase anchor protein, 350kDa, protein kinase A anchoring protein 9, centrosome- and golgi-localized protein. Official Symbol: AKAP9. RefSeq number(s): NP_671700.1, NP_005742.4, NP_671714.1. Gene ontology terms: centrosome, cytoskeleton, protein binding, receptor binding, signal transduction, synaptic transmission, transport

Specificity: This antibody is expected to recognise isoforms 1, 2 and 3 (NP_671700.1, NP_005742.4, NP_671714.1 respectively). The isoforms Yotiao, AKAP350B and AKAP350C are not recognized. Human, Mouse 3907 aa, 1655 aa, 3911 aa, 3899 aa

Synonyms: PRKA9, CG-NAP, YOTIAO, AKAP350, AKAP450, HYPERION, KIAA0803, MU-RMS-40.16A, AKAP-9, C79026, AW545847, mKIAA0803, 5730481H23Rik, Gisp, Yotiao, AKAP9, wu:fj02h02, prka9, yotiao, akap350, akap450, hyperion, AKAP18, Akap7, Akap15, Akap18, AKAP18d, MGC112559, AKAP15, AI662165, BB170514, 6430401D08, akap15, akap18, MGC83920, AKAP7, MGC139521

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 450kDa band observed in lysates of cell line HepG2 (calculated MW of 454kDa according to NP_ 671700.1). Recommended concentration: 1-3µg/ml. Immunohistochemistry: The staining pattern observed was identical to that obtained with a rabbit polyclonal antibody specific to AKAP350A (to be submitted to a scientific journal).

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Ciampi, Knauf, Kerler et al.: "Oncogenic AKAP9-BRAF fusion is a novel mechanism of MAPK pathway activation in thyroid cancer." in: The Journal of clinical investigation, Vol. 115, Issue 1, pp. 94-101, 2005 (PubMed).