Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS) antibody

Antigen: Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS)

Synonyms: THC, IMD2, THC1, WASP

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185368

Additional Information

Alternative name: Wiskott-Aldrich Syndrome / WASP

Gene ID: 7454

Immunogen: C-SPADKKRSGKKKI

Format: Affinity-purified

Description: All names and symbols: WAS, THC, IMD2, WASP, Wiskott-Aldrich syndrome (eczema-thrombocytopenia), thrombocytopenia 1 (X-linked), Wiskott-Aldrich syndrome (eczema-thrombocytopenia) protein. Official Symbol: WAS. RefSeq number(s): NP_000368.1. Gene ontology terms: actin cytoskeleton, actin polymerization and/or depolymerization, blood coagulation, defense response, epidermis development, protein complex assembly, small GTPase regulatory/interacting protein activity

Specificity: No cross-reactivity expected with N WASP (WASL). Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 60kDa band observed in Human lysates of Tcell line MOLT4 and monocytic cell line U937 (calculated MW of 52.9kDa according to NP_000368.1). Recommended concentration: 0.03-0

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185368 staining (0.03 ug/ml) of U937 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Yamaguchi, Lorenz, Kempiak et al.: "Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2/3 complex pathway and cofilin." in: The Journal of cell biology, Vol. 168, Issue 3, pp. 441-52, 2005 (PubMed).