Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) antibody

Antigen: Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1)

Synonyms: B23, NPM, MGC104254, Npm, NO38, MGC102162, MGC107291, B23NP, MGC108517, npm1, MGC53901, npm

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185372

Additional Information

Alternative name: Nucleophosmin / NPM1

Gene ID: 4869

Immunogen: C-QEAIQDLWQWRKSL

Format: Affinity-purified

Description: All names and symbols: NPM1, NPM, B23, nucleophosmin (nucleolar phosphoprotein B23, numatrin), numatrin, nucleolar phosphoprotein B23, nucleophosmin/nucleoplasmin family, member 1. Official Symbol: NPM1. RefSeq number(s): NP_002511.1, NP_954654.1. Gene ontology terms: NF-kappaB binding, RNA binding, Tat protein binding, activation of NF-kappaB transcription factor, cell aging, centrosome, centrosome cycle, cytoplasm, intracellular protein transport, negative regulation of cell proliferation, nucleocytoplasmic transport, nucleolus, protein heterodimerization activity, protein homodimerization activity, response to stress, ribosome assembly, transcription coactivator activity, unfolded protein binding

Specificity: This antibody is expected to recognise both report isoforms (NP_002511.1, NP_954654.1). Human, Mouse, Rat, Dog, Chimpanzee 294 aa, 295aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:2,000. Western Blot: Approx 37kDa band observed in Human HeLa lysates (calculated MW of 32.6kDa according to NP_002511.1 and 29.5kDa according to NP_954654.1). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185372 (0.1µg/ml) staining of Hela lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Grisendi, Pandolfi: "NPM mutations in acute myelogenous leukemia." in: The New England journal of medicine, Vol. 352, Issue 3, pp. 291-2, 2005 (PubMed).

Zhang, Garcia-Gras, Wycuff et al.: "Identification of direct serum-response factor gene targets during Me2SO-induced P19 cardiac cell differentiation." in: The Journal of biological chemistry, Vol. 280, Issue 19, pp. 19115-26, 2005 (PubMed).