NPM1 antibody (AA 212-292)

Catalog No. ABIN7438103

This Rabbit Polyclonal antibody specifically detects NPM1 in WB, IHC, IF and ICC. It exhibits reactivity toward Mouse.

Quick Overview for NPM1 antibody (AA 212-292) (ABIN7438103)

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-NPM1 Antibody

Binding Specificity: AA 212-292

Purpose: Polyclonal Antibody to Nucleophosmin 1 (NPM1)

Specificity: The antibody is a rabbit polyclonal antibody raised against NPM1. It has been selected for its ability to recognize NPM1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Nucleophosmin 1 (NPM1) corresdonding to Asp212~Leu292 (Accession # Q61937)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-30 μg/mL,Immunofluorescence:5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for NPM1

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Alternative Name: NPM1

Background: B23, NPM, Nucleolar Phosphoprotein B23, Numatrin, Nucleophosmin/Nucleoplasmin Family,Member 1, Nucleolar protein NO38

UniProt: Q61937

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly