»
Antibodies
»
anti-BH3 Interacting Domain Death Agonist (BID) antibody
BH3 Interacting Domain Death Agonist (BID) antibody
| Antigen |
|
| Synonyms |
FP497, MGC15319, MGC42355, AI875481, AU022477, 2700049M22Rik, BID, MGC140420, bid, si:ch211-238n5.6, xbid, fp497, DKFZp469E066 |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
Western Blotting (WB) (74), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (25), Flow Cytometry (FACS) (24), Immunoprecipitation (IP) (23), Immunofluorescence (IF) (21), Enzyme Immunoassay (EIA) (20), ELISA (19), Immunohistochemistry (IHC) (10), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
|
| Catalog no. |
ABIN185380 |
| Quantity |
100 ug (Variants) |
| Price |
501.80 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 Business Days |
|
Alternative name
|
BID
|
|
Gene ID
|
637
|
|
Immunogen
|
C-TYVRSLARNGMD
|
|
Format
|
Affinity-purified
|
|
Description
|
All names and symbols: BID, BH3 interacting domain death agonist , HGNC:1050, MGC15319, MGC42355 , BH3-interacting domain death agonist, Human BID coding sequence, apoptic death agonist. Official Symbol: BID. RefSeq number(s): NP_932070.1, NP_001187.1, NP_932071.1
|
|
Specificity
|
This antibody is expected to recognise all three reported isoforms (as represented by NP_932070.1, NP_001187.1, NP_932071.1) Human 241 aa, 195 aa, 99 aa
|
|
Application Notes
|
Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 26+24kDa bands observed in Human fibroblast A431 lysates (calculated MW of 26.8kDa according to NP_932070.1 and 22.0kDa according to NP_001187.1). Recommended concentration: 0.1-0.3µg/ml.
|
|
Purification
|
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
|
|
Buffer
|
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
|
|
Storage
|
Aliquot and store at -20°C. Minimize freezing and thawing.
|
|
Research Area
|
Cancer
|
|
Restrictions
|
For Research Use only
|
|
Publications
|
Lund, Stokke, Olsen et al.: "Garlic arrests MDA-MB-435 cancer cells in mitosis, phosphorylates the proapoptotic BH3-only protein BimEL and induces apoptosis." in: British journal of cancer, Vol. 92, Issue 9, pp. 1773-81, 2005 (PubMed).
|
Alternatives for antigen "BH3 Interacting Domain Death Agonist (BID)", type "Antibodies"
|
Hosts
|
Rabbit (64), Mouse (17), Goat (6), Rat (4)
|
|
Reactivities
|
Human (78), Mouse (Murine) (42), Rat (Rattus) (23), Dog (Canine) (5), Chicken (2), Pig (Porcine) (2), Cow (Bovine) (1), Sheep (Ovine) (1)
|
|
Applications
|
Western Blotting (WB) (74), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (25), Flow Cytometry (FACS) (24), Immunoprecipitation (IP) (23), Immunofluorescence (IF) (21), Enzyme Immunoassay (EIA) (20), ELISA (19), Immunohistochemistry (IHC) (10), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
|
|
Conjugates
|
Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
|
|
Epitopes
|
C-Term (5), pSer65 (2), BH3 Domain Specific (1), S61 (1), pS65 (1), pSer61 (1)
|
Alternatives 
