Pyruvate Dehydrogenase Complex, Component X (PDHX) antibody

Antigen: Pyruvate Dehydrogenase Complex, Component X (PDHX)

Synonyms: E3BP, OPDX, PDX1, proX, DLDBP, E3bp, Pdx1, AI481367, Ac1164, MGC66110, zgc:66110, zgc:85977, MGC86218, PDHX, MGC143185

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185389

Additional Information

Alternative name: Pyruvate dehydrogenase / PDHX

Gene ID: 8050, 27402 (mouse)

Immunogen: C-KSFKANLENPIRLA

Format: Affinity-purified

Description: All names and symbols: PDHX, pyruvate dehydrogenase complex, component X, E3BP, OPDX, PDX1, proX, DLDBP, E3-binding protein, pyruvate dehydrogenase complex, lipoyl-containing component X. Official Symbol: PDHX. RefSeq number(s): NP_003468.1. Gene ontology terms: acyltransferase activity, electron transporter activity, metabolism, mitochondrion, protein binding, pyruvate dehydrogenase complex (sensu Eukaryota)

Specificity: Human, Mouse, Rat, Chimpanzee 501 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 50kDa band observed in Human Pancreas lysate (calculated MW of 54.2kDa according to NP_003468.1). Recommended concentration: 0.1-1µg/ml. Immunohistochemistry: In paraffin embedded Human Pancreas, shows cytoplasmic staining of acinar cells, islets of Langerhans and peri-pancreatic fat cells. Recommended concentration, 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185236 (1µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABIN185389 staining of paraffin embedded Human Pancreas. A) Microwaved antigen retrieval with citrate buffer pH 6 (3µg/ml), HRP-staining. B) Microwaved antigen retrieval with Tris/EDTA buffer pH9 (1µg/ml) , HRP-staining.

Publications

Publications: Roche, Baker, Yan et al.: "Distinct regulatory properties of pyruvate dehydrogenase kinase and phosphatase isoforms." in: Progress in nucleic acid research and molecular biology, Vol. 70, pp. 33-75, 2001 (PubMed).