Reticulon 4 Receptor (RTN4R) antibody

Antigen: Reticulon 4 Receptor (RTN4R)

Synonyms: NGR, NOGOR, NgR, NgR1, Nogor

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185403

Additional Information

Alternative name: Nogo 66 receptor

Gene ID: 65078

Immunogen: C-LQALPDDTFRDLGN

Format: Affinity-purified

Description: All names and symbols: Nogo-66 receptor, RTN4R, reticulon 4 receptor , HGNC:1860, NGR, NOGOR, UNQ330/PRO526, nogo receptor, reticulon 4 receptor precursor. Official Symbol: RTN4R. RefSeq number(s): NP_075380.1

Specificity: Human, Mouse, Rat, Dog 473 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: experiments gave bands at approx 90-100kDa and 37+40+45kDa in Human brain lysate after 0.3µg/ml antibody staining. These bands were successfully blocked by the immunizing peptide and they correspond to earlier findings in the literature with different antibodies (Wang et al, J Neurosci. 2002 Jul 1,22(13):5505-15). This protein has a calculated MW of 50.7kDa according to NP_075380.1. Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185403 (0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Trifunovski, Josephson, Ringman et al.: "Neuronal activity-induced regulation of Lingo-1." in: Neuroreport, Vol. 15, Issue 15, pp. 2397-400, 2005 (PubMed).

Shao, Browning, Lee et al.: "TAJ/TROY, an orphan TNF receptor family member, binds Nogo-66 receptor 1 and regulates axonal regeneration." in: Neuron, Vol. 45, Issue 3, pp. 353-9, 2005 (PubMed).