Free Fatty Acid Receptor 1 (FFAR1) antibody

Antigen: Free Fatty Acid Receptor 1 (FFAR1)

Synonyms: Gpr40, FFAR1, GPR40

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185404

Additional Information

Alternative name: GPR40

Gene ID: 2864

Immunogen: CAARTQGGKSQK

Format: Affinity-purified

Description: All names and symbols: GPR40, G protein-coupled receptor 40 , HGNC:4498. Official Symbol: GPR40. RefSeq number(s): NP_005294.1

Specificity: Human 300 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 28kDa band observed in Human Breast lysates (calculated MW of 31.5kDa according to NP_005294.1). Recommended concentration: 0.01-0.03µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185404 (0.01µg/ml) staining of Human Breast lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Hardy, St-Onge, Joly et al.: "Oleate promotes the proliferation of breast cancer cells via the G protein-coupled receptor GPR40." in: The Journal of biological chemistry, Vol. 280, Issue 14, pp. 13285-91, 2005 (PubMed).