CCKBR antibody (Internal Region)

Catalog No. ABIN185407

This Goat Polyclonal antibody specifically detects CCKBR in WB, ELISA and IHC. It exhibits reactivity toward Human and Mouse and has been mentioned in 4+ publications.

Quick Overview for CCKBR antibody (Internal Region) (ABIN185407)

Target: CCKBR (Cholecystokinin B Receptor (CCKBR))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CCKBR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-CCKBR Antibody

Binding Specificity: Internal Region

Purpose: CCKBR

Sequence: FDGDSDSDSQ SRVRNQ

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-FDGDSDSDSQSRVRNQ, from the internal region of the protein sequence according to NP_795344.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Pancreas. Recommended concentration: 4-6 μg/mL.

Western Blot: Approx 48-50 kDa band observed in Mouse Pancreas lysates (calculated MW of 49.2 kDa according to Mouse NP_031653.1) Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-CCKBR antibody (ABIN185407)

Quattrone, Dewaele, Wozniak, Bauters, Vanspauwen, Floris, Schöffski, Chibon, Coindre, Sciot, Debiec-Rychter: "Promoting role of cholecystokinin 2 receptor (CCK2R) in gastrointestinal stromal tumour pathogenesis." in: The Journal of pathology, Vol. 228, Issue 4, pp. 565-74, (2014) (PubMed).

El-Kouhen, Morisset: "Control of somatostatin (SS) secretion by CCK-1 and CCK-2 receptors' occupation in RIN-14B cells, a rat pancreatic islet cell line." in: Pancreas, Vol. 39, Issue 2, pp. 127-34, (2010) (PubMed).

Berna, Seiz, Nast, Benten, Bläker, Koch, Lohse, Pace: "CCK1 and CCK2 receptors are expressed on pancreatic stellate cells and induce collagen production." in: The Journal of biological chemistry, Vol. 285, Issue 50, pp. 38905-14, (2010) (PubMed).

El-Kouhen, Morisset: "Cholecystokinin and Somatostatin Negatively Affect Growth of the Somatostatin-RIN-14B Cells." in: International journal of endocrinology, Vol. 2009, pp. 875167, (2009) (PubMed).

Target Details for CCKBR

Target: CCKBR (Cholecystokinin B Receptor (CCKBR))

Alternative Name: CCKBR

Background: CCKBR, cholecystokinin B receptor , HGNC:1571, CCK-B, GASR, CCK2 receptor, cholecystokinin-B receptor/gastrin receptor, gastrin receptor, gastrin\cholecystokinin brain receptor, CCK2R

Gene ID: 887, 12426, 25706

NCBI Accession: NP_795344

Pathways: Positive Regulation of Peptide Hormone Secretion, Feeding Behaviour