NPY5R antibody (Internal Region)

Catalog No. ABIN185409

This anti-NPY5R antibody is a Goat Polyclonal antibody detecting NPY5R in WB, IHC, ELISA and IP. Suitable for Human and Hamster. This Primary Antibody has been cited in 2+ publications.

Quick Overview for NPY5R antibody (Internal Region) (ABIN185409)

Target: NPY5R (Neuropeptide Y Receptor Y5 (NPY5R))

Reactivity: Human, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This NPY5R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Grade: Verified

Product Details anti-NPY5R Antibody

Binding Specificity: Internal Region

Purpose: Neuropeptide Y receptor Y5

Sequence: GLSNKENRLE EN

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GLSNKENRLEEN, from the internal region of the protein sequence according to NP_006165.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Intestine shows staining of ganglion cells. Recommended concentration, 4-6 μg/mL.

Western Blot: Approx 50 kDa band observed in Human Brain, Breast and Duodenum lysates (calculated MW of 50.7 kDa according to NP_006165.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in W

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunoprecipitation: Customer feedback showed specific results when this product was used in IP. This antibody has been successfully used in IP: PMID: 30503694.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-NPY5R antibody (ABIN185409)

Czarnecka, Kitlinska: "Cell Surface Protein Detection to Assess Receptor Internalization." in: Bio-protocol, Vol. 6, Issue 20, (2016) (PubMed).

Sheriff, Ali, Yahya, Haider, Balasubramaniam, Amlal: "Neuropeptide Y Y5 receptor promotes cell growth through extracellular signal-regulated kinase signaling and cyclic AMP inhibition in a human breast cancer cell line." in: Molecular cancer research : MCR, Vol. 8, Issue 4, pp. 604-14, (2010) (PubMed).

Target Details for NPY5R

Target: NPY5R (Neuropeptide Y Receptor Y5 (NPY5R))

Alternative Name: NPY5R

Background: Neuropeptide Y receptor Y5 , HGNC:7958, NPY5R, NPYR5

Molecular Weight: 50.7kDa

Gene ID: 4889, 18168, 25340

NCBI Accession: NP_006165

Pathways: Feeding Behaviour