PPY antibody (Internal Region)

Catalog No. ABIN185420

The Goat Polyclonal anti-PPY antibody has been validated for WB, IHC, ELISA, FACS and IF. It is suitable to detect PPY in samples from Human and Rat. There is 1 publication available.

Quick Overview for PPY antibody (Internal Region) (ABIN185420)

Target: PPY (Pancreatic Polypeptide (PPY))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This PPY antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-PPY Antibody

Binding Specificity: Internal Region

Purpose: Pancreatic Polypeptide / PPY

Sequence: TRPRYGKRHK EDT

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TRPRYGKRHKEDT, from the internal region of the protein sequence according to NP_002713.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans. Recommended concentration: 3 μg/mL. Paraffin embedded Human Pancreas and Intestine. Recommended co

Western Blot: This antibody has been successfully used in WB on Human, PMID: 25445712.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: This antibody has been successfully used in IF on Human, PMID: 27300574 and 30687234, and on Rat, PMID: 27472443.

Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-PPY antibody (ABIN185420)

Md Moin, Cory, Ong, Choi, Dhawan, Butler, Butler: "Pancreatic Nonhormone Expressing Endocrine Cells in Children With Type 1 Diabetes." in: Journal of the Endocrine Society, Vol. 1, Issue 5, pp. 385-395, (2017) (PubMed).

Target Details for PPY

Target: PPY (Pancreatic Polypeptide (PPY))

Alternative Name: PPY

Background: PPY, pancreatic polypeptide, HGNC:9327, PNP, pancreatic polypeptide Y

Gene ID: 5539

NCBI Accession: NP_002713

Pathways: Feeding Behaviour