TRPC6 antibody (C-Term)

Catalog No. ABIN185460

The Goat Polyclonal anti-TRPC6 antibody (ABIN185460) specifically detects TRPC6 in WB, ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for TRPC6 antibody (C-Term) (ABIN185460)

Target: TRPC6 (Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TRPC6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-TRPC6 Antibody

Binding Specificity: C-Term

Purpose: TRPC6

Sequence: EKLSMEPNQE ETN

Cross-Reactivity: Cow, Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EKLSMEPNQEETN, from the C Terminus of the protein sequence according to NP_004612.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 100 kDa band observed in lysates of cell line A549 (calculated MW of 106 kDa according to NP_004612.2). Preliminary experiments also showed 100 kDa in Human Lung lysates and in lysates of cell line HEK293.This molecular weight is routin

Peptide ELISA: antibody detection limit dilution 1:2000.

Comment:

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TRPC6

Target: TRPC6 (Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6))

Alternative Name: TRPC6

Background: TRPC6, transient receptor potential cation channel, subfamily C, member 6, HGNC:12338, TRP6, transient receptor potential channel 6, FLJ11098, FLJ14863, FSGS2, TRP6

Gene ID: 7225

NCBI Accession: NP_004612