Plasminogen Activator, Urokinase (PLAU) antibody

Antigen: Plasminogen Activator, Urokinase (PLAU)

Synonyms: ATF, UPA, URK, u-PA, uPA, UPAM, MGC124931, PLAU, MGC139972, LOC100008633, plau, DKFZp469F0732

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185470

Additional Information

Alternative name: Plasminogen Activator / PLAU

Gene ID: 5328

Immunogen: C-HFLPWIRSHTKEEN

Format: Affinity-purified

Description: All names and symbols: PLAU, plasminogen activator, urokinase, HGNC:9052, ATF, UPA, URK, u-PA , U-plasminogen activator, antagonist of uPA, plasminogen activator, urinary, urokinase plasminogen activator, urokinase-type plasminogen activator amino-terminal fragment, urokinase-type plasminogen activator precursor. Official Symbol: PLAU. RefSeq number(s): NP_002649.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 48kDa band observed in lysates of human kidney fibroblast cell line HEK293 (calculated MW of 48.5kDa according to NP_002649.1). Recommended concentration: 0.3-1.0µg/ml. A minor band was also consistently observed at appox 35kDa. This band was successfully blocked by incubation with the immunising peptide, and is consistent with the B chain of Cathepsin B-mediated cleavage product of PLAU (Kobayashi et al, J Biol Chem. 1991 Mar 15,266(8):5147-52, PMID: 1900515).

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185470 (0.3µg/ml) staining of 293 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Morgan, Hill: "Human breast cancer cell-mediated bone collagen degradation requires plasminogen activation and matrix metalloproteinase activity." in: Cancer cell international, Vol. 5, Issue 1, pp. 1, 2005 (PubMed).