Golgi Autoantigen, Golgin Subfamily A, 3 (GOLGA3) antibody

Antigen: Golgi Autoantigen, Golgin Subfamily A, 3 (GOLGA3)

Synonyms: MEA-2, GCP170, Mea2, Mea-2, AI449376, AW490576, 5330413L04, 5430416E01Rik, si:dkey-215k6.6

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185473

Additional Information

Alternative name: GOLGA3

Gene ID: 2802

Immunogen: C-HSQSRASKEGPGE

Format: Affinity-purified

Description: All names and symbols: GOLGA3, HGNC:4426, GCP170, MEA-2 , Golgi autoantigen, golgin subfamily a, 3, Golgi complex-associated protein of 170 kD, Golgi membrane associated protein, Golgi peripheral membrane protein, SY2/SY10 protein, golgin-160, golgin-165, male enhanced antigen-2. Official Symbol: GOLGA3. RefSeq number(s): NP_005886.2

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 170kDa band observed in human HeLa lysates (calculated MW of 167kDa according to NP_005886.2). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at appox 25kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN185473 (0.1µg/ml) staining of Hela lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Hicks, Machamer: "The NH2-terminal domain of Golgin-160 contains both Golgi and nuclear targeting information." in: The Journal of biological chemistry, Vol. 277, Issue 39, pp. 35833-9, 2002 (PubMed).