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phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) antibody
| Antigen | phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) |
| Synonyms | DBI, IBP, MBR, PBR, BZRP, PKBS, PTBR, mDRC, pk18, PEPCK, PEPCK2, PEPCK-M, 1810010O14Rik, 9130022B02Rik, PCK2, pck, zgc:77867, id:ibd5157, ppc, SCE66.06c, pepC, pckA |
| Clonality | Polyclonal |
| Host |
 Alternatives Goat |
| Reactivity |
Alternatives Human, Mouse (Murine), Rat (Rattus) |
| Application |
Alternatives ELISA, Western Blotting (WB)
|
 |
1 reference available |
| Catalog no. | ABIN185483 |
| Quantity | 100 ug |
| Price | 501.80 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business Days |
Additional Information
| Alternative name | PCK2 / PEPCK-M |
| Gene ID | 5106 |
| Immunogen | KPWKPGDKEPCAH |
| Format | Affinity-purified |
| Description | All names and symbols: PCK2, PEPCK-M, phosphoenolpyruvate carboxykinase 2 (mitochondrial), HGNC:8725, PEPCK, PEPCK2 , PEP carboxykinase, mitochondrial phosphoenolpyruvate carboxykinase 2, phosphoenolpyruvate carboxylase, phosphopyruvate carboxylase. Official Symbol: PCK2. RefSeq number(s): NP_004554.2. Gene ontology terms: obesity |
| Specificity | This antibody is expected to recognise isoform 1 (NP_004554) only, no cross-reactivity with PCK1. Human, Mouse, Rat, Dog, Cow |
Application Details
| Application Notes | Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 75kDa band observed in human liver lysates (calculated MW of 70.7kDa according to NP_004554.2). Recommended concentration: 0.5-1.0 µg/ml. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Restrictions | For Research Use only |
Images
| ABIN185483 (0.5µg/ml) staining of human liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
Publications
| Publications |
Duplus, Benelli, Reis et al.: "Expression of phosphoenolpyruvate carboxykinase gene in human adipose tissue: induction by rosiglitazone and genetic analyses of the adipocyte-specific region of the promoter in type 2 diabetes." in: Biochimie, Vol. 85, Issue 12, pp. 1257-64, 2004 (PubMed).
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Alternatives
Alternatives for antigen "phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK)", type "Antibodies"
