PEPCK antibody (AA 1-640)

Catalog No. ABIN7599509

The Mouse Monoclonal anti-PEPCK antibody is suitable to detect PEPCK in samples from Human, Mouse, Rat and Monkey. It has been validated for WB, IHC, FACS, IF and ICC.

Quick Overview for PEPCK antibody (AA 1-640) (ABIN7599509)

Target: PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This PEPCK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 3F7

Product Details anti-PEPCK Antibody

Binding Specificity: AA 1-640

Purpose: Anti-PCK2 Antibody (monoclonal, 3F7)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PCK2 Antibody.(monoclonal, 3F7)-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PCK2 recombinant protein (Position: M1-M640).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat, Monkey Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PEPCK

Target: PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

Alternative Name: PCK2

Background:

Background: Phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2, PEPCK-M), is an isozyme of phosphoenolpyruvate carboxykinase (PCK, PEPCK) that in humans is encoded by the PCK2 gene. It is mapped to 14q11.2-q12. This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.

Gene Full Name: phosphoenolpyruvate carboxykinase 2, mitochondrial

Molecular Weight: 71 kDa

Gene ID: 5106

UniProt: Q16822

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis