Eukaryotic Translation Initiation Factor 3, Subunit M (EIF3M) antibody

Antigen: Eukaryotic Translation Initiation Factor 3, Subunit M (EIF3M)

Synonyms: B5, GA17, PCID1, hfl-B5, FLJ29030, Ga17, Pcid1, MGC118449, RGD1565840, pcid1, fa16c10, zgc:63996, wu:fa16c10, wu:fc41f09, ga17, tango7, MGC52639, hfl-b5, MGC69424, HFL-B5, MGC127047, DKFZp459D197

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185490

Additional Information

Alternative name: B5 Receptor / PCID1 / EIF3M

Gene ID: 10480

Immunogen: C-NAWKQNLNKVKN

Format: Affinity-purified

Description: All names and symbols: B5 receptor, PCID1, PCI domain containing 1 (herpesvirus entry mediator), FLJ29030, GA17, hfl-B5, dendritic cell protein, fetal lung protein B5. Official Symbol: EIF3M. RefSeq number(s): NP_006351.2

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 38-40kDa band observed in lysates of human lung carcinoma cell line A549 (calculated MW of 42.5kDa according to NP_006351.2). Recommended concentration: 1-3µg/ml. Immunohistochemistry: In paraffin embedded Human Testis shows strong staining of cytoplasm in select spermatocytes. Recommended concentration, 5-10µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185490 (0.1µg/ml) staining of A549 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABIN185490 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Perez-Romero, Fuller: "The C terminus of the B5 receptor for herpes simplex virus contains a functional region important for infection." in: Journal of virology, Vol. 79, Issue 12, pp. 7431-7, 2005 (PubMed).