Chromobox Homolog 3 (CBX3) antibody

Antigen: Chromobox Homolog 3 (CBX3)

Synonyms: MGC94711, Hp1-gamma, MGC105792, MGC105793, MGC156678, CBX3, HECH, HP1-GAMMA, HP1Hs-gamma

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185493

Additional Information

Alternative name: CBX3 / HP1 Gamma

Gene ID: 11335

Immunogen: C-EAFLNSQKAGKEKD

Format: Affinity-purified

Description: All names and symbols: CBX3, HP1-GAMMA, chromobox homolog 3 (HP1 gamma homolog, Drosophila) , HGNC:1553, HECH, HP1Hs-gamma , HP1 gamma homolog, chromobox homolog 3, chromobox homolog 3 (Drosophila HP1 gamma), heterochromatin protein HP1 gamma, heterochromatin-like protein 1. Official Symbol: CBX3. RefSeq number(s): NP_009207.2, NP_057671.2

Specificity: No cross-reactivity expected with HP1-alpha and HP1-beta. NP_009207.2 and NP_057671.2 represent same protein. Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx. 20kDa band observed in lysates of A431, HeLa and HepG2. (calculated MW of 20.8kDa according to NP_009207.2 and NP_057671.2). Recommended concentration: 0.3-1.0 µg/ml. Immunohistochemistry: In paraffin embedded Human Placenta shows strong nuclear staining in trophoblasts. Recommended concentration, 2-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185493 (0.3µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABIN185493 (2.5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Vakoc, Mandat, Olenchock et al.: "Histone H3 lysine 9 methylation and HP1gamma are associated with transcription elongation through mammalian chromatin." in: Molecular cell, Vol. 19, Issue 3, pp. 381-91, 2005 (PubMed).