TIRAP antibody (Internal Region)

Catalog No. ABIN185499

The Goat Polyclonal anti-TIRAP antibody (ABIN185499) specifically detects TIRAP in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for TIRAP antibody (Internal Region) (ABIN185499)

Target: TIRAP (Toll-Interleukin 1 Receptor (TIR) Domain Containing Adaptor Protein (TIRAP))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TIRAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-TIRAP Antibody

Binding Specificity: Internal Region

Purpose: TIRAP

Sequence: QTLLKKPKKR PNSPE

Specificity: This antibody is expected to recognize both reported isoforms (NP_001034750.1 and NP_683708.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QTLLKKPKKRPNSPE, from the internal region of the protein sequence according to NP_001034750.1, NP_683708.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 22-23 kDa band observed in lysates of cell lines A549 and K562 and in Human colorectal cancer lysates (calculated MW of 23.9 kDa according to NP_001034750.11). Recommended concentration: 0.1-0.5 μg/mL. Primary incubation 1 hour at room

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TIRAP

Target: TIRAP (Toll-Interleukin 1 Receptor (TIR) Domain Containing Adaptor Protein (TIRAP))

Alternative Name: TIRAP

Background: TIRAP, toll-interleukin 1 receptor (TIR) domain containing adaptor protein, HGNC:17192, FLJ42305, Mal, wyatt, MyD88 adapter-like protein, TIR domain-containing adapter protein, Toll-interleukin 1 receptor (TIR) domain-containing adapter protein, Toll-inte

Gene ID: 114609

NCBI Accession: NP_001034750, NP_683708

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades