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Antibodies
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anti-BR serine/threonine Kinase 2 (BRSK2) antibody
BR serine/threonine Kinase 2 (BRSK2) antibody
| Antigen |
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| Synonyms |
SAD1, STK29, PEN11B, C11orf7, FLJ41362, SADA, SAD-A, 4833424K13Rik, BRSK2 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)
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Application
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| Catalog no. |
ABIN185503 |
| Quantity |
100 ug |
| Price |
261.61 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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BRSK2 / STK29
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Gene ID
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9024, 75770 (mouse), 293631 (rat)
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Immunogen
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CPEVIRGEKYDGRKAD
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Description
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All names and symbols: BRSK2, SAD1, STK29, PEN11B, C11orf7, BR serine/threonine kinase 2, serine/threonine kinase 29, homolog of SAD-1 (C. elegans), chromosome 11 open reading frame 7, chromsosome 11 open reading frame 7. Official Symbol: BRSK2. RefSeq number(s): NP_003948.2
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Specificity
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Human, Mouse, Rat, Dog 668 aa
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Preliminary experiments gave bands at approx 50-55kDa and 15kDa in human brain, mouse brain and rat brain lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 74.7kDa according to NP_003948.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Restrictions
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For Research Use only
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Kishi, Pan, Crump et al.: "Mammalian SAD kinases are required for neuronal polarization." in: Science (New York, N.Y.), Vol. 307, Issue 5711, pp. 929-32, 2005 (PubMed).
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Alternatives for antigen "BR serine/threonine Kinase 2 (BRSK2)", type "Antibodies"
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Hosts
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Rabbit (37), Goat (2)
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Reactivities
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Human (37), Mouse (Murine) (26), Rat (Rattus) (22), Chicken (19), Cow (Bovine) (19), Dog (Canine) (19), Horse (Equine) (18), Pig (Porcine) (18), Sheep (Ovine) (18), Cat (Feline) (1)
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Applications
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Western Blotting (WB) (21), ELISA (20), Immunofluorescence (IF) (16), Immunohistochemistry (IHC) (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)
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Epitopes
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C-Term (7), Internal Region (2), Center (1), Internal Region,AA 328-356 (1)
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Alternatives 
