Monoamine Oxidase B (MAOB) antibody

Antigen: Monoamine Oxidase B (MAOB)

Synonyms: MGC26382, MAO-B, 6330414K01Rik, MAOB, MGC139719, MAOA, Maob, DKFZp459N0430, maob, Z-MAO, zgc:85761, wu:fb68b05, wu:fo76d11, wu:fq38g06

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185506

Additional Information

Alternative name: MAOB

Gene ID: 4129, 109731 (mouse)

Immunogen: C-HKARKLARLTKEE

Format: Affinity-purified

Description: All names and symbols: MAOB, monoamine oxidase B , HGNC:6834, MGC26382, RP1-201D17__B.1, MAO, brain, MAO, platelet, adrenalin oxidase, amine oxidase (flavin-containing), tyramine oxidase. Official Symbol: MAOB. RefSeq number(s): NP_000889.3

Specificity: This antibody is not expected to cross-react with MAOA. Human, Mouse, Rat, Dog,

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 60kDa band observed in Human Brain lysates (calculated MW of 58.8kDa according to NP_000889.3). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185506 (1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Domschke, Sheehan, Lowe et al.: "Association analysis of the monoamine oxidase A and B genes with attention deficit hyperactivity disorder (ADHD) in an Irish sample: preferential transmission of the MAO-A 941G allele to affected children." in: American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, Vol. 134B, Issue 1, pp. 110-4, 2005 (PubMed).