Klotho antibody (Internal Region)

Catalog No. ABIN185513

The Goat Polyclonal anti-Klotho antibody has been validated for IHC and ELISA. It is suitable to detect Klotho in samples from Human.

Quick Overview for Klotho antibody (Internal Region) (ABIN185513)

Target: Klotho (KL)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Klotho antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Klotho Antibody

Binding Specificity: Internal Region

Purpose: Klotho

Sequence: KRDDAKYMYY LKK

Specificity: This antibody is expected to recognise both reported isoforms (NP_004786.2, NP_710150.1).

Predicted Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KRDDAKYMYYLKK, from the internal region of the protein sequence according to NP_004786.2, NP_710150.1.

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Preliminary experiments in Human Brain, Duodenum, Placenta and Prostate lysates gave no specific signal but low background at antibody concentration up to 1 μg/mL.

DS IHC Results: In paraffin embedded Human Breast shows cytoplasm staining in luminal epithelial cells of ducts, while Human Placenta shows membranous staining. Recommended concentration: 4-6 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Klotho

Target: Klotho (KL)

Alternative Name: Klotho

Background: KL, klotho

Gene ID: 9365

NCBI Accession: NP_004786, NP_710150

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Activity, Growth Factor Binding