Klotho antibody (Middle Region)

Catalog No. ABIN2776875

This Rabbit Polyclonal antibody specifically detects Klotho in WB and IHC. It exhibits reactivity toward Mouse, Human, Rat, Rabbit, Horse, Cow, Dog and Guinea Pig.It has been mentioned in 1 publication

Quick Overview for Klotho antibody (Middle Region) (ABIN2776875)

Target: Klotho (KL)

Reactivity: Mouse, Human, Rat, Rabbit, Horse, Cow, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Klotho antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Klotho Antibody

Binding Specificity: Middle Region

Purpose: KL Antibody - middle region

Sequence: HAQNGKISIA LQADWIEPAC PFSQKDKEVA ERVLEFDIGW LAEPIFGSGD

Predicted Reactivity: Cow: 79%, Dog: 86%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against KL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human KL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP41554-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Klotho antibody (ABIN2776875)

Ritter, Zhang, Delmez, Finch, Slatopolsky: "Differential expression and regulation of Klotho by paricalcitol in the kidney, parathyroid, and aorta of uremic rats." in: Kidney international, Vol. 87, Issue 6, pp. 1141-52, (2016) (PubMed).

Target Details for Klotho

Target: Klotho (KL)

Alternative Name: KL

Background:

Background Information: KL is a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss.This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Klotho

Alternative Symbols: HFTC3

Protein Name: Klotho

Molecular Weight: 116kDa

Gene ID: 9365

NCBI Accession: NP_004786

UniProt: Q9UEF7

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Activity, Growth Factor Binding