Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila) (ROBO1) (internal) antibody

Antigen: Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila) (ROBO1)

Synonyms: SAX3, DUTT1, FLJ21882, MGC131599, MGC133277, Gm310, AW494633, AW742721, ROBO1, robo1, sax3, dutt1, MGC122264, roundabout, roundabout-1

Epitope: internal

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185537

Additional Information

Alternative name: ROBO1 / DUTT1 (Internal)

Gene ID: 6091, 19876 (mouse), 58946 (rat)

Immunogen: C-GDVDLSNKINEMK

Format: Affinity-purified

Description: All names and symbols: ROBO1, DUTT1, roundabout, axon guidance receptor, homolog 1 (Drosophila) , HGNC:10249, FLJ21882, SAX3, axon guidance receptor, roundabout 1. Official Symbol: ROBO1. RefSeq number(s): NP_002932.1, NP_598334.1

Specificity: This antibody is expected to recognise both reported isoforms (NP_002932.1, NP_598334.1) Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 250kDa band observed in Human Endothelial Cell line HUVEC lysates (calculated MW of 181kDa according to NP_002932.1). Some of the observed proteolytic breakdown products are also found in Human, Mouse and Rat Brain lysates. Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185537 (1µg/ml) staining of HUVEC lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Hannula-Jouppi, Kaminen-Ahola, Taipale et al.: "The axon guidance receptor gene ROBO1 is a candidate gene for developmental dyslexia." in: PLoS genetics, Vol. 1, Issue 4, pp. e50, 2005 (PubMed).