Vacuolar Protein Sorting 25 Homolog (S. Cerevisiae) (VPS25) antibody

Antigen: Vacuolar Protein Sorting 25 Homolog (S. Cerevisiae) (VPS25)

Synonyms: DERP9, EAP20, FAP20, MGC10540, AW107467, D11Wsu68e, 1110020N13Rik, VPS25, dVps25, l(2)44Db, l(2)k08904, DmelCG14750, CG14750, vps25, MGC162560, im:6898134, MGC86224

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185544

Additional Information

Alternative name: VPS25

Gene ID: 84313

Immunogen: C-QPNVDTRQKQ

Description: All names and symbols: VPS25, vacuolar protein sorting 25 (yeast), HGNC:28122, DERP9, EAP20, MGC10540 , vacuolar protein sorting 25. Official Symbol: VPS25. RefSeq number(s): NP_115729.1

Specificity: Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:1,000. Western Blot: Approx 20-22kDa kDa band observed in human duodenum, kidney and liver lysates (calculated MW of 20.7kDa according to NP_115729.1). Recommended concentration: 0.5-1.5 µg/ml. Immunohistochemistry: In paraffin embedded human kidney, shows granular cytoplasmic staining of endothelial cells. Recommended concentration, 10µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN185544 (0.5 ug/ml) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABIN185544 (10 ug/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Publications

Vaccari, Bilder: "The Drosophila tumor suppressor vps25 prevents nonautonomous overproliferation by regulating notch trafficking." in: Developmental cell, Vol. 9, Issue 5, pp. 687-98, 2005 (PubMed).

Thompson, Mathieu, Sung et al.: "Tumor suppressor properties of the ESCRT-II complex component Vps25 in Drosophila." in: Developmental cell, Vol. 9, Issue 5, pp. 711-20, 2005 (PubMed).