TRIM5 antibody (Internal Region)

Catalog No. ABIN185553

This anti-TRIM5 antibody is a Goat Polyclonal antibody detecting TRIM5 in ELISA and IHC. Suitable for Human.

Quick Overview for TRIM5 antibody (Internal Region) (ABIN185553)

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TRIM5 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-TRIM5 Antibody

Binding Specificity: Internal Region

Purpose: TRIM5 / RNF88 (alpha isoform)

Sequence: CNIEKNENYQ PK

Specificity: This antibody is expected to recognise isoform alpha (NP_149023.1) only.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CNIEKNENYQPK, from the internal region of the protein sequence according to NP_149023.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows cytoplasm staining in PCT. Recommended concentration, 3-6 μg/mL.

Western Blot: Preliminary experiments gave an approx 37 kDa band in Lysates of Human cell lines Daudi, Jurkat and MOLT4 after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe g

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TRIM5

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Alternative Name: TRIM5

Background: TRIM5, tripartite motif-containing 5 , HGNC:16276, RNF88, tripartite motif protein 5, tripartite motif protein TRIM5, TRIM5alpha, OTTHUMP00000069812, tripartite motif protein TRIM, tripartite motif-containing protein 5 alpha

Gene ID: 85363

NCBI Accession: NP_149023

Pathways: Activation of Innate immune Response