Intraflagellar Transport 88 Homolog (Chlamydomonas) (IFT88) antibody

Antigen: Intraflagellar Transport 88 Homolog (Chlamydomonas) (IFT88)

Synonyms: DAF19, TG737, TTC10, hTg737, MGC26259, D13S1056E, RP11-172H24.2, fxo, orpk, Tg737, Ttc10, flexo, polaris, AW552028, Tg737Rpw, TgN737Rpw, 146590_s_at, CG12548, CG14465, CG15190, CG4137, DmelCG12548, IFT88, nomp, NOMPB, osm-5, OSM-5

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185555

Additional Information

Alternative name: IFT88 / Polaris

Gene ID: 8100, 21821 (mouse), 305918 (rat)

Immunogen: C-KKRIDEDDFADEE

Format: Affinity-purified

Description: All names and symbols: IFT88, polaris, TTC10, tetratricopeptide repeat domain 10, HGNC:20606, D13S1056E, DAF19, IFT88, MGC26259, RP11-172H24.2, TG737, hTg737 , TPR repeat protein 10, Probe hTg737 (polycystic kidney disease, autosomal recessive), Tg737 protein, intraflagellar transport 88 homolog, recessive polycystic kidney disease protein Tg737 homolog. Official Symbol: IFT88. RefSeq number(s): NP_783195.2, NP_006522.2

Specificity: This antibody is expected to recognise both reported isoforms (NP_783195.2, NP_006522.2) Human, Mouse, Rat, Dog

Synonyms: DAF19, TG737, TTC10, hTg737, MGC26259, D13S1056E, RP11-172H24.2, fxo, orpk, Tg737, Ttc10, flexo, polaris, AW552028, Tg737Rpw, TgN737Rpw, 146590_s_at, CG12548, CG14465, CG15190, CG4137, DmelCG12548, IFT88, nomp, NOMPB, osm-5, OSM-5

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments in Human Kidney, Liver and Lung lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Immunohistochemistry: In paraffin embedded Human Kidney shows staining of the brush border in PCT. Recommended concentration, 3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185555 (3µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Publications

Publications: Vogel: "News focus: Betting on cilia." in: Science (New York, N.Y.), Vol. 310, Issue 5746, pp. 216-8, 2005 (PubMed).

Miyoshi, Kasahara, Miyazaki et al.: "Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2009 (PubMed).