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CD14 Molecule (CD14) antibody

Antigen

CD14 Molecule (CD14)
Synonyms CD14
Clonality Polyclonal
Host
Reactivity
Alternatives

Human
Application
Alternatives ELISA, Western Blotting (WB)
2 references available
Catalog no. ABIN185563
Quantity 100 ug  (Variants)
Price 501.80 $   Plus shipping costs $35.00
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anti-CD14 Molecule (CD14) antibody

Additional Information
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Alternative name CD14
Gene ID 929
Immunogen C-KRVDADADPRQYAD
Format Affinity-purified
Description All names and symbols: CD14, CD14 antigen , HGNC:1628. Official Symbol: CD14. RefSeq number(s): NP_000582.1
Specificity Human 375 aa

Application Details
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Application Notes Peptide ELISA: antibody detection limit dilution 1:128,000. Approx 55kDa band observed in Human Lung, Lymph Node and Tonsil lysates (calculated MW of 40.1kDa according to NP_000582.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Funda et al, Infect Immun. 2001 Jun,69(6):3772-81, PMID: 11349042). Recommended concentration: 1-3µg/ml.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Research Area Stem Cells, Innate Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Immunology, Inflammation
Restrictions For Research Use only

Publications
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Publications Kim, Lee, Jin et al.: "Crystal structure of CD14 and its implications for lipopolysaccharide signaling." in: The Journal of biological chemistry, Vol. 280, Issue 12, pp. 11347-51, 2005 (PubMed).

Iwaki, Nishitani, Mitsuzawa et al.: "The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain." in: Biochemical and biophysical research communications, Vol. 328, Issue 1, pp. 173-6, 2005 (PubMed).

Alternatives
Application Details Publications Images Secondary Antibodies Variants back to top

Alternatives for antigen "CD14 Molecule (CD14)", type "Antibodies"
Hosts Mouse (241), Rabbit (36), Goat (7), Rat (2), Chicken (1)
Reactivities Human (265), Pig (Porcine) (34), Mouse (Murine) (30), Cow (Bovine) (28), Dog (Canine) (26), Sheep (Ovine) (25), Rabbit (24), Monkey (23), Primates (19), Rat (Rattus) (18), Goat (9), Cat (Feline) (8), Primate (8), Chicken (1), Llama (1)
Applications Flow Cytometry (FACS) (218), Western Blotting (WB) (54), ELISA (36), Immunoprecipitation (IP) (32), Functional Studies (Func) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (24), Immunohistochemistry (Frozen Sections) (IHC (fro)) (22), Immunofluorescence (IF) (20), Enzyme Immunoassay (EIA) (19), Immunohistochemistry (IHC) (16), ELISA (Detection) (5), Blocking Antibody (Inhibition) (3), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Radioimmunoassay (RIA) (1)
Conjugates FITC (35), PE (24), Biotin (21), APC (16), RPE (8), Cy5.5 (5), Alexa Fluor 488 (3), Pacific Blue (3), Alexa Fluor 700 (2), Cy5 (2), Cy7,PE (2), PE,Cy5 (2), PE,Cy7 (2), PE,DY590 (2), PE,DY647 (2), SPRD (2), APC,Cy7 (1), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), CF (1), Cy3 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5.5 (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), PerCP (1), RBITC (1), RPE,Cy5 (1), RPE,Cy5.5 (1)
Epitopes C-Term (2), N-Term (2)